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LOCALIZATION OF PROTEOGLYCANS IN THE CELL NUCLEUS OF SEA URCHINS AS REVEALED BY MEANS OF LECTIN-BINDING AND NUCLEASE DIGESTION
https://saitama-med.repo.nii.ac.jp/records/111
https://saitama-med.repo.nii.ac.jp/records/111e07e9374-2d01-4a66-8653-53db17fee3d0
名前 / ファイル | ライセンス | アクション |
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KJ00000101479.pdf (1.8 MB)
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
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公開日 | 1998-03-31 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | LOCALIZATION OF PROTEOGLYCANS IN THE CELL NUCLEUS OF SEA URCHINS AS REVEALED BY MEANS OF LECTIN-BINDING AND NUCLEASE DIGESTION | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ページ属性 | ||||||
内容記述タイプ | Other | |||||
内容記述 | P(論文) | |||||
著者名(英) |
KINOSHITA,S./YOSHII,K.
× KINOSHITA,S./YOSHII,K. |
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著者所属(英) | ||||||
言語 | en | |||||
値 | Department of Biology, Saitama Modical School/Nursing School, St. Marianna University, School of Medicine | |||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | The distribution of proteoglycans in the embryonic cell nucleus of sea urchins was studied by means of lectin binding using Ricinus communis agglutinin I (RCA-120) and other lectins. By electron microscopy using avidin-biotin complex technique, the localization of nuclear proteoglycans was found to be restricted exclusively to areas of dispersed chromation, i.e. euchromatic regions, in the uncleus. Fluorometric determination of lectin binding ability onto the partially digested chromatin using FITC-labeled lectins showed that lectin binding sites were exposed by the action of pancreatic DNase-I but remained masked by Staphylococcal nuclease. Considering the difference of the mode of action of these enzymes, it is likely that the lectin binding sites are more abundant in the regions of active genes than those of inactive ones. The pattern of localization of the nuclear proteoglycans suggests a possible participation of these substances in the mechanism of gene activation. | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00095070 | |||||
書誌情報 |
埼玉医科大学進学課程紀要 巻 7, p. 1-12, 発行日 1998-03-31 |