{"created":"2023-06-19T10:21:39.072463+00:00","id":625,"links":{},"metadata":{"_buckets":{"deposit":"a2ed46c3-f67d-460b-96fd-0cecad901e62"},"_deposit":{"created_by":12,"id":"625","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"625"},"status":"published"},"_oai":{"id":"oai:saitama-med.repo.nii.ac.jp:00000625","sets":["6:10:90"]},"author_link":["2167","2166"],"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-03-23"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"埼玉医科大学"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学） "}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"平成29年度","subitem_description_type":"Other"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Activated factor X (FXa) plays an important role in thrombin generation and inflammation. Factor X is not converted constitutively to FXa, but only after intrinsic clotting factors are activated and/or cellular injury occurs. Although rivaroxaban is one of direct FXa inhibitors, its function in the inactivated coagulation cascade is unclear. In human umbilical vein endothelial cells that natively express protease-activated receptor-1 and -2, high dose rivaroxaban did not alter gene transcripts including pro-inflammatory genes in DNA microarray. Upon FXa stimulation, the expressions of pro-inflammatory genes such as monocyte chemoattractant protein-1 (MCP-1), intracellular adhesion molecule-1, and interleukin-8 were maximally increased at 4 h after stimulation, and were suppressed by rivaroxaban. To confirm these results, quantitative polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA) for MCP-1 were performed. FXa evoked the expression of MCP-1 maximally at 4 h after stimulation, whereas MCP-1 displayed a different temporal activation in ELISA. Interestingly, rivaroxaban inhibited both time courses of MCP-1 expression. These results suggest that rivaroxaban may not influence gene modulation in the inactivated coagulation state, but can attenuate the endothelial damage evoked by FXa and pro-inflammatory cytokine genes.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"32409甲第1373号"}]},"item_10006_relation_14":{"attribute_name":"掲載誌名","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Journal of Pharmacological Sciences"}]}]},"item_10006_relation_15":{"attribute_name":"掲載誌情報","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"出版社サイト "}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/pii/S1347861317300257?via%3Dihub","subitem_relation_type_select":"URI"}}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"関, 要"},{"creatorName":"セキ, カナメ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2166","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Seki, Kaname","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2167","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-05-30"}],"displaytype":"detail","filename":"1373-1.pdf","filesize":[{"value":"103.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"論文内容の要旨","url":"https://saitama-med.repo.nii.ac.jp/record/625/files/1373-1.pdf"},"version_id":"f794e87c-f359-4df3-9190-96d2d859e3bf"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-05-30"}],"displaytype":"detail","filename":"1373-2.pdf","filesize":[{"value":"128.4 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"論文審査の結果の要旨","url":"https://saitama-med.repo.nii.ac.jp/record/625/files/1373-2.pdf"},"version_id":"2967b2cd-3101-4b09-afa2-b48a02945add"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cells, Cultured","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Chemokine CCL2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Factor Xa","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Factor Xa Inhibitors","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Human Umbilical Vein Endothelial Cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Humans","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Intercellular Adhesion Molecule-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Interleukin-8","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Oligonucleotide Array Sequence Analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Receptors, Proteinase-Activated","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rivaroxaban","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"凝固第X因子活性時 / 非活性時におけるRivaroxabanの振る舞い","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"凝固第X因子活性時 / 非活性時におけるRivaroxabanの振る舞い"},{"subitem_title":"Demeanor of rivaroxaban in activated/inactivated FXa.","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"12","path":["90"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-05-30"},"publish_date":"2018-05-30","publish_status":"0","recid":"625","relation_version_is_last":true,"title":["凝固第X因子活性時 / 非活性時におけるRivaroxabanの振る舞い"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2023-06-19T10:34:01.475801+00:00"}